For many years, scientists have been on a quest to identify a non-invasive cancer biomarker – a biological molecule found in the blood that indicates the presence of disease. Their hope was that it would provide a more effective and patient-friendly method for the detection, monitoring and treatment of cancer. However, due to the very nature of cancer, identifying an effective biomarker has proven to be quite a challenge. Now, with the recent advances in sequencing technology, a solution has been found. A large number of cancer genome sequencing studies have collectively identified the genetic changes that make human tumors grow and progress. As a result of their findings, scientists have discovered that virtually all cancers carry somatic DNA mutations. Unlike hereditary mutations that are passed from parent to child and are present in every cell in the body, somatic mutations form in the DNA of individual cells during a person’s life. Because these somatic mutations are only present in tumor cell DNA, they provide an extremely specific biomarker that can be detected and tracked. Although a tumor itself is the major source of tumor DNA, acquiring DNA through a biopsy is invasive, risky and often not possible. Fortunately, scientists have discovered that dying tumor cells release small pieces of their DNA into the bloodstream. These pieces are called cell-free circulating tumor DNA (ctDNA). February’s Genome Advance of the Month describes a new study, published in the February 19, 2014, issue of Science Translational Medicine, which examines the potential of screening ctDNA for somatic mutations as a way to detect and follow the progression of a patient’s tumor. The team of scientists began by identifying at least one somatic mutation present in the tumors of 187 patients with 17 different types of advanced cancer. To do this, they used a tiered approach for each patient: they started by sequencing several genes commonly mutated in cancer, and if no mutation was found, they widened their search and sequenced all of the protein coding regions of the genome or the entire genome itself. The ctDNA present in the patients’ blood was then examined for the specific somatic mutation identified in their tumor. The scientists were able to detect the ctDNA somatic mutation for 82 percent of the patients with metastatic tumors outside the brain. In comparison, 55 percent of patients with early stages of cancer had detectable levels of ctDNA mutations in their blood. From these findings, they discovered that the percentage of patients with detectable levels of ctDNA in their blood correlated with the stage of cancer. While only 47 percent of patients with stage I cancers had detectable ctDNA, the percentage of patients with stage II, III and IV cancers was 55, 69 and 82 percent, respectively. Moreover, the scientists found that the concentration of ctDNA in blood increased as the cancer stage increased. This suggests that simply measuring the level of ctDNA in a patient’s blood could be used in the future as a way to determine how advanced their cancer is. In fact, when the scientists measured the ctDNA concentration in 206 colorectal cancer patients, they saw that patients with lower blood levels of ctDNA lived significantly longer than those with higher levels of ctDNA. In addition to its potential role as a detection and prognostic method, ctDNA was also evaluated as a way of monitoring tumor progression and testing whether a patient’s tumor would respond to targeted drug treatments. The scientists examined the ctDNA present in the blood of 24 colorectal cancer patients whose tumors had first responded to a specific gene targeted therapy, but then progressed while still being treated. The patients’ ctDNA was screened for mutations both before and after therapy. The scientists found new somatic mutations that prevent the drug from working formed during the patients’ treatment. Because the patients saw an initial tumor response to the drug, this suggests that the drug was initially effective in killing tumor cells but that the formation of the new mutations stopped the drug from continuing to work. This valuable information would indicate to doctors that the patients’ tumors are no longer responsive and different treatment is necessary. The authors of this study demonstrated that ctDNA testing could be applied to every stage of cancer patient care. They showed that ctDNA is detected in most types of cancer at both early and advanced stages, suggesting it could be used as an effective screening method for most patients. A measurement of the levels of ctDNA in blood may also be used to quickly estimate a patient’s stage of cancer and survival chances. Finally, the authors exhibited the utility of ctDNA in monitoring the response of tumors to treatment and determining which drugs may be effective in the future. Overall, ctDNA appears to be an extremely effective and advantageous biomarker. Because it is found in the blood, it provides a non-invasive, and thus less risky, alternative method to repeated tumor biopsies to monitor tumor progression. While further studies will need to be performed before it reaches the clinic, the evidence provided in this study demonstrates the immense potential of ctDNA to improve the early detection and treatment of cancer.
月度归档:2016年05月
Wako 和光纯药 Phos-tag磷酸化蛋白研究 Phos-tag™ Acrylamide AAL-107
Nard
——————
–
301-93531
Phos-tag™ Biotin BTL-104
Phos-tag™ 生物素
– 10 mg 8,570.00 –
Wako
——————
和光纯药
193-16571
SuperSep™ Phos-tag™ (50 μmol/L), 12.5%,17 well
Phos-tag™ 预制胶50μmol/l,12.5%,17孔
– 5块 3,370.00 –
Wako
——————
和光纯药
193-16691
SuperSep™ Phos-tag™ (50 μmol/L), 15%,13 well
Phos-tag™ 预制胶50μmol/l,15%,13孔
– 5块 3,370.00 –
Manac
——————
–
302-93561
Phos-tag™ Agarose
Phos-tag™ 琼脂糖
– 0.5 ml 2,450.00 –
Nard
——————
–
387-07321-8P
Phos-tag™ Tip
Phos-tag™ 琼脂糖吸头
– 8 pcs 2,470.00 –
Nard
——————
–
304-93521
Phos-tag™ Acrylamide AAL-107
Phos-tag™ 丙烯酰胺
– 10 mg 7,340.00 –
Nard
——————
–
304-93526
Phos-tag™ Acrylamide AAL-107 5mM Aqueous Solution
Phos-tag™ 丙烯酰胺5mM水溶液
– 0.3 ml 1,840.00 –
Wako
——————
和光纯药
038-23221
α-Casein, from Bovine Milk, Dephosphorylated
牛奶源去磷酸化α酪蛋白
for Biochemistry 1 mg 570.00 –
Nard
——————
–
305-93551
Phos-tag™ Mass Analytical Kit
Phos-tag™ 质谱分析试剂盒
– 1 set 咨询 –
Wako
——————
和光纯药
058-07681
EasySeparator
Phos-tag预制凝胶的配套电泳槽
for Electrophoresis 1 set 5,950.00 –
Manac
——————
–
308-93563
Phos-tag™ Agarose
Phos-tag™ 琼脂糖
– 3 ml 12,000.00 –
Nard
——————
–
308-97201
Phos-tag™ Biotin BTL-111 1mM Aqueous Solution
Phos-tag™ 生物素1mM水溶液
– 0.1 ml 2,450.00 –
Wako
——————
和光纯药
190-16721
SuperSep™ Phos-tag™ (50 μmol/L), 10%,17 well
Phos-tag™ 预制胶50μmol/l,10%,17孔
– 5块 3,370.00 –
Wako
——————
和光纯药
198-17981
SuperSep™ Phos-tag™ (50μmol/l), 7.5%, 17well, 83×100×3.9mm
Phos-tag™ 预制胶50μmol/l,7.5%,17孔,BioRad型
for Electrophoresis 5 EA 3,370.00 –
Wako
——————
和光纯药
195-17991
SuperSep™ Phos-tag™ (50μmol/l), 12.5%, 17well, 83×100×3.9mm
Phos-tag™ 预制胶50μmol/l,12.5%,17孔,BioRad型
for Electrophoresis 5 EA 3,370.00 –
Wako
——————
和光纯药
192-18001
SuperSep™ Phos-tag™ (50μmol/l), 7.5%, 17well, 100×100×6.6mm
Phos-tag™ 预制胶50μmol/l,7.5%,17孔,Life Technologies型
for Electrophoresis 5 EA 3,370.00 –
Wako
——————
和光纯药
199-18011
SuperSep™ Phos-tag™ (50μmol/l), 12.5%, 17well, 100×100×6.6mm
Phos-tag™ 预制胶50μmol/l,12.5%,17孔,Life Technologies型
for Electrophoresis 5 EA 3,370.00 –
Wako
——————
和光纯药
195-16391
SuperSep™ Phos-tag™ (50 μmol/L), 12.5%,13 well
Phos-tag™ 预制胶50μmol/l,12.5%,13孔
– 5块 3,370.00 –
Nard
——————
–
387-07321
Phos-tag™ Tip
Phos-tag™ 琼脂糖吸头
– 8 pcs ×10 23,900.00 –
Nard
——————
–
386-15221
Phos-tagTM Magenta
Phos-tagTM 品红荧光染料
– 0.2 mg 2,240.00
LabAssay 磷脂检测试剂盒 296-63801 1300 tests –
LabAssay Phospholipid
LabAssay 磷脂检测试剂盒
品牌:Wako
CAS No.: 储存条件:2-10℃ 纯度:– |
产品编号(生产商编号) | 等级 | 规格 | 运输包装 | 零售价(RMB) | 库存情况 | 参考值 |
296-63801 | for Cellbiology | 1300 tests | – | 5610 | – | – |
* 干冰运输、大包装及大批量的产品需酌情添加运输费用
检测样品:血清。
检测范围:0~596.1mg/dL
检测灵敏度:纯水时,OD=0.14;OD=0.12~0.58,浓度为300mg/dL
相关动物模型:肝脏疾病
检测原理:胆碱氧化酶 ・ DAOS法
测定的是卵磷脂,溶血卵磷脂,鞘磷脂三者的总和。
Wako 和光纯药 10%硝酸银埋藏硅胶 233-01611 二噁英分析试剂
——————
和光纯药
136-13461
Methanol
甲醇
for Dioxins Analysis 1 L 咨询 67-56-1
Wako
——————
和光纯药
197-11611
10% Silver Nitrate-impregnated Silica Gel
10%硝酸银埋藏硅胶
for Dioxins Analysis 100 g 咨询 N/A
Wako
——————
和光纯药
233-01611
Wakosil DNPH Eluent A
DNPH洗脱液A
for High Performance Liquid Chromatography 1 L 560.00 N/A
Wako
——————
和光纯药
011-17741
Acetonitrile
乙腈
for Aldehyde Analysis 200 ml 咨询 (75-05-8)
Wako
——————
和光纯药
233-02191
Wakosil DNPH-II Eluent B
DNPH-II 洗脱液B
for High Performance Liquid Chromatography 1 L 720.00 N/A
Wako
——————
和光纯药
237-61733
Wakopak Wakosil-DNPH-II 4.6*150mm
醛酮类物质分析色谱柱
No grade 1 column(W) 6,730.00 N/A
Wako
——————
和光纯药
238-01781
Wakogel DX
硅胶Wakogel® DX
for Dioxins Analysis 100 g 咨询 7631-86-9
Wako
——————
和光纯药
201-15541
2,2′,3-Trihydroxydiphenyl Ether Standard
2,2,3’-三羟基联苯酯标准品
Wako Special Grade 100 mg 咨询 91368-55-7
Wako
——————
和光纯药
132-13463
Methanol
甲醇
for Dioxins Analysis 3 L 咨询 67-56-1
Wako
——————
和光纯药
016-17833
Acetone
丙酮
for Dioxins Analysis 3 L 咨询 67-64-1
Wako
——————
和光纯药
040-27481
Dioxin Trap Beads
二噁英吸附填料
for Dioxins Analysis 200 g 咨询 N/A
Wako
——————
和光纯药
297-43051
Presep® Acitive Carbon-impregnated Silica Gel(Reverse Column)
活性炭埋藏硅胶反相柱(二噁英前处理柱)
for Dioxins Analysis 5 pcs 2,060.00 –
Wako
——————
和光纯药
048-28543
Decane
癸烷
for Dioxins Analysis 2 ml×5 咨询 124-18-5
Wako
——————
和光纯药
236-02181
Wakosil DNPH-II Eluent A
DNPH-II 洗脱液A
for High Performance Liquid Chromatography 1 L 720.00 N/A
Wako
——————
和光纯药
019-11941
Active Carbon-impregnated Silica Gel
活性炭埋藏硅胶
for Dioxins Analysis 10 g 咨询 –
Wako
——————
和光纯药
042-28541
Decane
癸烷
for Dioxins Analysis 100 ml 咨询 124-18-5
Wako
——————
和光纯药
083-07391
Hexane
己烷
for Dioxins Analysis 1 L 咨询 110-54-3
Wako
——————
和光纯药
167-19251
2% Potassium Hydroxide-impregnated Silica Gel
2%氢氧化钾埋藏硅胶
for Dioxins Analysis 100 g 咨询 N/A
Wako
——————
和光纯药
238-59411
Wakopak Wakosil-DNPH 4.6*250mm
醛酮类物质分析色谱柱
No grade 1 column(D) 7,290.00 N/A
Wako
——————
和光纯药
050-06661
Ethanol(99.5)
乙醇(99.5)
for Dioxins Analysis 1 L 咨询 64-17-5
日本 三博特 Sanplatec iP-TEC活细胞运输中间容器套装
——————
三博特
WEB28445
iP-TEC flask -25 (P-25)
iP-TEC flask-25培养瓶 (P-25)30ml
– 100个 咨询 –
Sanplatec
——————
三博特
WEB28448
iP-TEC miniscraper (150-3)
iP-TEC 迷你刮刀 (150-3)
– 100个 咨询 –
Sanplatec
——————
三博特
WEB28451
secondary container of Live transport set (Sheet,Flask pallet ,Box)
活细胞运输中间容器套装(吸液片6个、专用托盘2个、盒子1个)
– 1 set 咨询 –
Sanplatec
——————
三博特
WEB28452
Pallet of Live transport
活细胞运输专用托盘
– 12 pcs 咨询 –
Sanplatec
——————
三博特
WEB28453
Liquid absorbing sheet
吸液片
– 36 pcs 咨询 –
Sanplatec
——————
三博特
WEB28481
iP-TEC premium box-V8.5 set ( box,t heat storage-36 5pcs)
iP-TEC精装版保温运输箱 V8.5 套装 (箱子,36-蓄热板5块)
– 1 set 咨询 –
Sanplatec
——————
三博特
WEB28482
iP-TEC premium box-V8.5 (box only)
iP-TEC精装版保温运输箱 V8.5(仅箱子)
– 1 pc 咨询 –
Sanplatec
——————
三博特
WEB28460
iP-TEC premium box-V19 (box only)
iP-TEC精装版保温运输箱 V19 (仅箱子)
– 1 pc 咨询 –
Sanplatec
——————
三博特
WEB28463
iP-TEC standard box-X13 (box only)
iP-TEC标准版保温运输箱 X13 (仅箱子)
– 1 pc 咨询 –
Sanplatec
——————
三博特
WEB28466
iP-TEC Light box-S6.6 ( box,t heat storage-36 2pcs)
iP-TEC轻便版保温运输箱 S6.6 (箱子,36-蓄热板2块)
– 1 set 咨询 –
Sanplatec
——————
三博特
WEB28462
iP-TEC premium box-V19set( box,t heat storage-36 6pcs)
iP-TEC精装版保温运输箱 V19套装(箱子,36-蓄热板6块)
– 1 set 咨询 –
Sanplatec
——————
三博特
WEB28465
iP-TEC standard box-X13set ( box,t heat storage-36 6pcs)
iP-TEC标准版保温运输箱 X13 套装(箱子,36-蓄热板6块)
– 1 set 咨询 –
Sanplatec
——————
三博特
WEB28483
iP-TEC latent heat storage material-24 (1 pc)
iP-TEC蓄热板-24(1块)
– 1 pc 咨询 –
Sanplatec
——————
三博特
WEB28486
iP-TEC premium box-V8.5 set ( box,t heat storage-24 5pcs)
iP-TEC精装版保温运输箱 V8.5 套装 (箱子,24-蓄热板5块)
– 1 set 咨询 –
Sanplatec
——————
三博特
WEB28484
iP-TEC premium box-V19 set ( box,t heat storage-24 6pcs)
iP-TEC精装版保温运输箱 V19 套装(箱子,24-蓄热板6块)
– 1 set 咨询 –
Sanplatec
——————
三博特
WEB28487
iP-TEC Light box-S6.6 ( box,t heat storage-24 2pcs)
iP-TEC轻便版保温运输箱 S6.6(箱子,24-蓄热板2块)
– 1 set 咨询 –
Sanplatec
——————
三博特
WEB28488
iP-TEC Cap for Microtiter plate suitable for 6wells
iP-TEC微量滴定板盖适合6孔
– 1 pc 咨询 –
Sanplatec
——————
三博特
WEB28489
iP-TEC Cap for Microtiter plate suitable for 6wells
iP-TEC微量滴定板盖适合6孔
– 10 pcs 咨询 –
Sanplatec
——————
三博特
WEB28490
iP-TEC Cap for Microtiter plate suitable for 12wells
iP-TEC微量滴定板盖适合12孔
– 1 pc 咨询 –
Sanplatec
——————
三博特
WEB28491
iP-TEC Cap for Microtiter plate suitable for 12wells
iP-TEC微量滴定板盖适合12孔
– 10 pcs 咨询
LabAssay肌酐检测试剂盒 290-65901 500 tests
LabAssay™ Creatinine
LabAssay肌酐检测试剂盒
品牌:Wako
CAS No.: 储存条件:2-10℃ 纯度:– |
产品编号(生产商编号) | 等级 | 规格 | 运输包装 | 零售价(RMB) | 库存情况 | 参考值 |
290-65901 | for Cellbiology | 500 tests | – | 3210 | – | – |
* 干冰运输、大包装及大批量的产品需酌情添加运输费用
检测样品:血清和尿液。
检测范围:0~10mg/dL
检测灵敏度:纯水时,OD=0.010~0.020;OD=0.400~0.500,浓度为10mg/dL
相关动物模型:肾脏疾病
检测原理:Jaffé法
Cell-Free DNA BCT
A Standardized Method for Sample Collection, Stabilization, and Transport of Cell-Free Plasma DNA
Cell-Free DNA BCT CE is a 10 mL blood collection tube with a formaldehyde-free preservative stabilizing nucleated blood cells. This unique stabilization, prevents the release of genomic DNA, allowing isolation of high-quality cell-free DNA which can be further used for a wide range of downstream applications commonly used in clinical research studies, drug discovery and diagnostic assay development.
Improved Logistics
Cell-Free DNA BCT CE reduces the need for immediate plasma preparation due to its unique stabilization properties. Cell-Free DNA is stable for up to 14 days, while circulating tumor cells are stable for up to 4 days, at room temperature, allowing convenient sample collection, transport and storage.
Cell-Free DNA BCT CE | Catalog Number |
---|---|
Cell-Free DNA BCT CE 6-Tube Pack – EXPORT ONLY | 218996 |
Cell-Free DNA BCT CE 100-Tube Box – EXPORT ONLY | 218997 |