标签归档：Hampton 蛋白质结晶

Hampton Polyethylene glycol 4,000

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Hampton Polyethylene glycol 4,000

Applications
Crystallization grade Polyethylene glycol for formulating screens or for optimizatio
Features
Sterile filtered solution
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)

Description
Polyethylene glycol 4,000
Synonym: PEG 4,000
H(OCH2CH2)nOH
Mr 3,500-4,500
CAS Number [25322-68-3]
EC Number 500-038-2
Merck 14,7568
RTECS TQ3500000
MDL Number MFCD00081839
PubChem Substance ID 24890217
Melting point (Starting Material) 58.0-61.0°C
Aldehyde None detected
Peroxide None detected

HR2-529 Measured pH range: 6.9 – 9.5 at 25°C
HR2-529 Measured Conductivity Range: 35.3 – 46.9 µS/cm at 25°C
HR2-529 Measured Refractive Index Range: 1.40172 – 1.40279 at 20°C

CAT NO NAME DESCRIPTION
HR2-605 Polyethylene glycol 4,000 Flake – 500 g
HR2-529 Polyethylene glycol 4,000 50% w/v solution – 200 ml

Hampton AlumaSeal™ II Sealing Film and Applicator

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Hampton AlumaSeal™ II Sealing Film and Applicator

Applications

Sealing film used to reseal HT format screen kits in polypropylene blocks and plates

Features

Excellent seal
Film conforms to raised chimney wells
Easily pierceable with single or multichannel pipettors and robotic probes
Heat & cold resistant, recommended for temperatures from -80 °C to +120 °C
Certified DNase-, RNase-, and nucleic-acid-free
Less evaporation than clear films
Excellent barrier properties, virtually no reagent evaporation or drying
 Description
A 38 µm soft non-permeable aluminum foil sealing film with strong medical-grade adhesive, AlumaSeal II sealing films eliminate the need for heat-sealing devices or mats during the resealing of reagents in polypropylene deep well blocks. Each sealing film measures 82.6 x 142.9 mm and offers sufficient sealing area for 96 deep well blocks. Length between the perforations with end tabs removed is 125.4 mm. Compared to other aluminum foils, AlumaSeal II has less tendency to roll back on itself when removing the backing paper and it conforms well to the plate during application.AlumaSeal II is a soft, pierceable adhesive film designed for the convenient and rapid sealing of polypropylene deep well blocks. A multiple split backing with two end tabs allows for easy, accurate positioning and secure sealing. The use of an adhesive sealing film minimizes evaporation and helps to prevent well-to-well cross contamination in reagent blocks. AlumaSeal II films are easily pierced by pipettte tips or robotic probes or piercing tools for direct reagent recovery without significant gumming by adhesive.

AlumaSeal™ II Sealing Film and Applicator

AlumaSeal™ II Sealing Film and Applicator

CAT NO NAME DESCRIPTION
HR8-069 AlumaSeal II Sealing Film 100 pack
HR4-413 Film Sealing Paddle 5 pack

Hampton CrystalEX Second Generation (Corning)

发表于由

Hampton CrystalEX Second Generation (Corning)

Crystal<i>EX</i> Second Generation (Corning)

CrystalEx Plates

Applications

   Sitting drop crystallization

Features

96 well sitting drop plate
Round and conical flat bottom drop well shapes
1, 2 or 4 microliter drop wells
1, 3 or 5 drop wells per reservoir
Hydrophilicity treated or untreated
PZero or COC plate material–UV imaging compatible

Description

The second generation of Corning® 96 Well, sitting drop format plates are built to SBS specifications, making them well suited for high throughput crystallization and are fully compatible with robotic equipment. The plates are available in several different configurations with varying drop well shapes, plate materials, and number of drop wells per reagent well. The basic plate design is one reagent well flanked by one or three drop wells, with SBS standard spacing between the centers of adjacent well clusters. One may choose a plate with small (1 µl), medium (2 µl), or large (4 µl) drop well volumes. The choice of round or conical flat well shapes are available. The PZero polymer provides for zero background polarization and is non-birefringent. PZero plates are not treated. The COC polymer offers high chemical resistance. Both types of plastic feature improved transparency. The reservoir numbers are embossed on each individual well for easy identification. Drop well locations conform to SBS standards for robotic handling. The low-volume reagent well saves on reagent costs. The plates can be sealed using Crystal Clear Sealing Film (HR3-609), 3 inch wide Crystal Clear Sealing Tape (HR4-506) or ClearSeal Film™ (HR4-521).

Corning has discontinued 3557 – special pricing and limited inventory available for this item only.

CAT NO NAME DESCRIPTION
HR8-135 Corning 3556 4µl round drop well, 1 drop well, COC, untreated – 10 plate case
HR8-134 Corning 3556 4 µl  round drop well, 1 drop well, COC, untreated – 40 plate case
HR8-137 Corning 3551 4 µl conical flat drop well, 1 drop well, COC, treated – 10 plate case
HR8-136 Corning 3551 4µl conical flat drop well, 1 drop well, COC, treated – 40 plate case
HR8-139 Corning 3552 2 µl round drop well, 3 drop well, COC, untreated – 10 plate case
HR8-138 Corning 3552 2µl  round drop well, 3 drop well, COC, untreated – 40 plate case
HR8-141 Corning 3553 2 µl conical flat drop well, 3 drop well, COC, untreated – 10 plate case
HR8-140 Corning 3553 2 µl conical flat drop well, 3 drop well, COC, untreated – 40 plate case
HR8-147 Corning 3550 1µll conical flat drop well, 3 drop well, COC, untreated – 10 plate case
HR8-146 Corning 3550 1µl conical flat drop well, 3 drop well, COC, untreated – 40 plate case
HR8-160 Corning 3557 1 µl conical flat drop well, 5 drop well, PZero – 10 plate case
HR8-158 Corning 3557 1 µl conical flat dro p well, 5 drop well, PZero – 40 plate case

Hampton Solubility & Stability Screen

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Hampton Solubility & Stability Screen

Solubility & Stability Screen（溶解度和稳定性的屏幕）

应用：溶解性和稳定性屏幕的设计，以协助识别解决方案的条件，促进蛋白质的溶解性和稳定性，并尽量减少蛋白质沉淀。

Solubility & Stability Screen

Solubility & Stability Screen

Solubility & Stability Screen

Solubility & Stability Screen HR2-072

Applications

A solubility screen, a stability screen, an additive screen for protein assays including ThermoFluor and crystallization
Preformulation screening

 

Compatible with Thermofluor , Differential Scanning Fluorimetry (DSF), and Protein Thermal Shift assays
96 sterile filtered reagents
17 classes of reagents and excipients
Highly concentrated (10x) reagent formulation
Developed at Hampton Research

Features

Methodologies

Thermal shift assay
Dynamic Light Scattering
Use Solubility & Stability Screen along side Slice pH and Solubility & Stability Screen 2 to optimize sample buffer
Description

Solubility & Stability Screen is designed to assist in the identification of solution conditions which promote protein solubility and stability, and minimize protein precipitation. Solubility & Stability Screen is a solubility screen, a stability screen, and may also be used as an additive screen in the presence of a crystallization reagent.

The Hampton Research Solubility and Stability Screen can evaluate protein solubility, stability and crystallization in the presence of 94 different chemical additives sampling 17 different classes of reagents plus two controls.

Protein solubility and stability are universally required in a wide range of applications, including general biochemical studies, the preparation of proteins in pharmaceuticals, structural biology and crystallization.1-11The preparation of a concentrated, soluble and stable protein sample can often be a difficult task as proteins often aggregate, precipitate or denature.

Thermofluor (also known as Differential Scanning Fluorimetry, DSF and Protein Thermal Shift) provides a rapid, convenient and effective means of identifying subsaturating solution conditions that may also correlate with protein heterogeneity and poor crystallization outcomes.12

Protein solubility and stability is affected by many different chemical factors including pH, buffer type, chemical additives and excipients. pH and buffer type are dominant protein solubility and stability variables and can be evaluated and optimized using the Hampton Research Slice pH kit HR2-070. Slice pH evaluates protein solubility, stability and crystallization versus 20 different buffers over the pH range 3.5-9.6. Chemical additives influencing protein solubility and stability can be evaluated using the Solubility & Stability Screen.

It is widely accepted that protein solubility and stability can be increased by the use of chemical additives.5,15Seventeen classes of reagents are sampled by the Solubility & Stability Screen and each of these classes has been reported as important in improving sample solubility and stability.2-11

The Solubility & Stability Screen is a set of 94 high purity reagents formulated in high purity water (NCCLS/ASTM Type 1+) at 25°C and are 0.22 micron sterile filtered. The 94 solubility and stability reagents are formulated at 2 to 10 times their recommended working concentration. The remaining two reagents are water and a negative (TCA) control. A water control demonstrates the effect of diluting sample as well as sample buffer concentration. TCA, the negative control, demonstrates total sample precipitation, loss of sample solubility and loss of sample stability. The effects of the Solubility & Stability reagents can be compared with this negative control to assist in the identification of reagents promoting sample solubility and stability. 500 microliters of each reagent is supplied in a sterile 96 well polypropylene Deep Well block. The Solubility & Stability Screen reagents are compatible with the sitting or hanging drop vapor diffusion, microbatch, free interface diffusion, sandwich drop vapor diffusion, and dialysis crystallization methods utilizing water soluble reagents.

Hampton 银弹 Silver Bullets

发表于由

Hampton 银弹 Silver Bullets

Applications

Additive screen for the optimization of protein crystals
For use with soluble proteins and membrane proteins
Additive screen to discover different crystal forms
Secondary or orthogonal crystallization screen when traditional screens are not successful
Additive screen for the optimization of protein solubility and stability

Features

Developed at Hampton Research
Screens a portfolio of small molecules and excipients for their ability to establish stabilizing, intermolecular, hydrogen bonding, hydrophobic and electrostatic interactions which could promote lattice formation and crystallization

Small organic acids & organic salts
Biologically active small molecules
Amino acids & peptides
Macromolecular digests
Co-factors & ligands
Biochemical pathway intermediates
Nucleotides, pharmacaphores & carbohydrates

Description
Silver Bullets screens a portfolio of small molecules for their ability to establish stabilizing, inter molecular, hydrogen bonding, hydrophobic and electrostatic interactions which could promote stability, lattice formation, and crystallization.1-3

Published results with the Silver Bullets have been very encouraging, with more than twice as many proteins being crystallized overall as were crystallized from controls free of any small molecules.1-3

X-ray diffraction analysis has revealed the small molecule Silver Bullets in the crystal lattice, involved at the centers of hydrogen bonding networks and electrostatic interaction.1-3 Silver Bullets is compatible with hanging, sitting and sandwich drop vapor diffusion, microbatch, free interface, and microdialysis crystallization methods. Silver Bullets can be used with Dynamic Light Scattering (DLS), ThermoFluor, and Size Exclusion Chromatography assays.

Silver Bullets reagent portfolio
•Organic salts and acids
•Biologically active small molecules
•Amino acids and peptides
•Macromolecular digests

The Silver Bullets kit is a library of small molecules that have been shown to promote crystal lattice formation. X-ray diffraction analysis has demonstrated the reagents have the ability to:

•Stabilize the conformation of the protein
•Perturb the interaction of the protein with the solvent
•Participate in forming important lattice contacts
•Build the crystal lattice by forming reversible cross-links between the macromolecules in the crystal

The Silver Bullets kit is composed of 96 solutions in a single Deep Well block (Greiner 786261 block specifications: Total Volume: 0.5 mL Working Volume: 0.03 – 0.7 mL at Room Temperature 0.03 – 0.55 mL at -20ºC, Well Profile: Conical (V), Bottom Well Bottom: Solid, Plate Color: Translucent), HT format.

Each Silver Bullets reagent is a mixture of small molecules or macromolecular digests in 0.02 M HEPES sodium pH 6.8 buffer. Each solution contains between 2 and 20 small molecules.

Silver Bullets reagent volume is 0.5 ml (each well).

ThermoFluor is a registered trademark of Johnson & Johnson.

Hampton  Silver Bullets Bio

Silver Bullets • Silver Bullets Bio

Silver Bullets • Silver Bullets Bio

上海金畔生物作为Hampton蛋白结晶产品的代理商，竭诚为您服务，欢迎新老客户咨询。

Applications 应用

Additive screen for the optimization of protein crystals
For use with soluble proteins and membrane proteins
Additive screen to discover different crystal forms
Secondary or orthogonal crystallization screen when traditional screens are not successful
Additive screen for the optimization of protein solubility and stability

Features

Screens a portfolio of small molecules and excipients for their ability to establish stabilizing, intermolecular, hydrogen bonding, hydrophobic and electrostatic interactions which could promote lattice formation and crystallization

Small organic acids & organic salts
Biologically active small molecules
Amino acids & peptides
Macromolecular digests
Co-factors & ligands
Biochemical pathway intermediates
Nucleotides, pharmacaphores & carbohydrates

 
Developed at Hampton Research
Two sets of 96 reagents, composed of more than 1,090 chemicals, of which more than 400 are uniqu

Please note, the Silver Bullets Bio Screen is temporarily unavailable as a new formulation is in development

 

Hampton 水晶透明密封带Crystal Clear Sealing Tape

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Hampton 水晶透明密封带Crystal Clear Sealing Tape

Crystal Clear Sealing Tape

3 inch, 1.88 inch, 1.88 inch with dispenser, 0.75 inch with dispenser (left to right)

Crystal Clear Sealing Tape

0.75 inch Crystal Clear Sealing Tape

Applications  应用
Sealing tape used to seal sitting drop crystallization experiments

Description

These optically clear tapes are compatible with protein crystallization reagents. The polypropylene Crystal Clear Sealing Tapes use acrylic solvent based adhesives which are compatible with aqueous crystallization reagents.

Catalog number HR3-511 is a 1.88 inch (48 mm) wide, 3 mil tape on a 43.7 yard (40 M) roll with a 1.5 inch core and is supplied with a green dispenser / cutter.Catalog number HR4-511 is a 1.88 inch wide, 3 mil tape on a 60 yard roll with a 3 inch core and no dispenser / cutter.Two strips of the HR3-511 or HR4-511 will seal a Corning, Cryschem, CrystalClear Strip, Greiner, Intelliplate, Linbro, Swissci (MRC), VDX and VDXm plate.Catalog number HR4-506 is a 3 inch wide, 3 mil tape on a 54.86 yard roll with a 3 inch core and no dispenser / cutter.One strip of the HR4-506 will seal a Corning, Cryschem M, CrystalClear Strip, Greiner, Intelliplate, Swissci (MRC) and VDXm plate.Crystal Clear Mini Sealing Tape HR4-508 is a 0.75 inch (19 mm) wide, 3 mil tape on a 650 inch (16.5 M) roll and is supplied with a clear dispenser / cutter. The tape is useful for resealing or patching wells after mounting crystals. The tape is also wide enough to seal a single row of a Cryschem S or Cryschem M plate.See chart for tape and plate compatibility.

Hampton热销产品

发表于由

Hampton Research是 美国最权威的蛋白结晶试剂盒提供商，产品齐全，性价比高。

在生命科学领域，上海金畔生物为业界提供了丰富的实验室和生物医药生产研发的产品。庆祝上海金畔生物正式成为Hampton的签约代理商，在这里要感谢广大客户多年来对上海金畔生物科技有限公司的支持和厚爱，我们将一如既往的为广大客户带来Hampton高品质的产品和服务，欢迎广大新老客户来电咨询。‘

美国Hampton Research公司位于加利福尼亚州，是一家专门从事蛋白质晶体研究的生产商。该公司向全球科研人员提供全面的晶体研究试剂和相关实验耗材，同时提供晶体设计和合成方面的定制服务。Hampton Research公司以其先进的生物大分子结晶技术和广泛实用的产品线，不仅为全世界晶体研究人员的工作带来了便捷，而且也受益于科研人员的回馈不断发展自己，目前已成为晶体研究领域最受信赖的品牌之一。

Hampton research主要产品：

Crystallization Screens 用于筛选和优化蛋白质/多肽/核酸结晶的一系列经验证的高精配方型溶液

Optimize Reagents 结晶级别盐类，聚合物，有机溶液或缓冲液

Crystallization Plates, Hardware & Accessories 结晶板（悬滴法和坐滴法，微量透析，微配液，自由界面扩散，凝胶以及无容器悬浮法）及其他配件

Cryocrystallography 低温晶体学耗材如 Crystalcap system,cryoloops

Goniometer Heads&Supplies 测角器头和配件

Protein Crystallization Standards 结晶级别标准蛋白

Custom Shop Crystallization Reagents 定制试剂盒和结晶溶液内的单一组分

StockOptions Kits 内含盐类，缓冲液和冷冻液的简便型试剂盒

Tools，Seeding&Resin 结晶用探针，结晶笔，seeding法结晶工具

Capillary Mounts & Supplies 毛细管,玻璃纤维, 蜡,黏合剂和密封剂

Hampton热销产品

  • StockOptions pH Buffer Kit HR2-241

    Crystallization grade buffer stocks for crystal screening, optimization, & production

    Crystal Screen Cryo HR2-122

    Primary screen with cryo for proteins, soluble peptides, nucleic acids, & water soluble small molecules

  • Natrix HR2-116

    Primary biased sparse matrix crystallization screen for nucleic acids & protein/nucleic acid complexes

  • MembFac HR2-114

    Primary sparse matrix crystallization screen for membrane proteins and samples with limited solubility

  • SaltRx HR2-136

    Primary or secondary, salt and pH matrix crystallization screen for biological macromolecules

  • Grid Screens HR2-248

    Primary or secondary, salt, polymer, organic and pH grid crystallization screen for biological macromolecules

  • PEG/Ion HR2-126

    Primary or secondary, polymer, salt and pH matrix crystallization screen for biological macromolecules

  • PCT™ Pre-Crystallization Test HR2-140

    Determine the appropriate sample concentration for crystallization screening

  • Index HR2-144

    Primary, diverse reagent system crystallization screen for proteins, complexes, peptides, nucleic acids, & water soluble small molecules

  • Crystal Screen HR2-110

    Primary screen for proteins, soluble peptides, nucleic acids, & water soluble small molecules;Sparse matrix additive screen

  • PEGRx HR2-082

    Primary and secondary, polymer and pH based crystallization screen for biological macromolecules

  • Additive Screen HR2-428

    Manipulate sample-sample & sample-solvent interactions to improve crystals or alter sample solubility

  • Detergent Screen HR2-408

    Prevent and manipulate non-specific aggregation due to hydrophobic interactions

  • CryoPro HR2-073

    Water soluble cryoprotectant reagent set designed for the cryopreservation of biological macromolecular crystals

  • StockOptions Salt HR2-245

    Crystallization grade salt reagent stock solutions for screen formulation and optimization

  • Siliconized Glass Cover Slides HR3-217

    Hanging, sitting or sandwich drop crystallization

  • Intelli-Plate 96 HR3-185

    蛋白结晶座滴板

  • CrystalCap HR4-733

  • CryoLoop HR4-623

    • CryoTong™ – Long HR5-104

      Crystal transfer under cryo temperature

    • CrystalWand™ Magnetic HR4-729

      CrystalCap Magnetic & CrystalCap Copper Magnetic handling tool

    • Vial Clamp™ – Straight HR4-670

      Vial support and manipulation

    • CrystalCap™ Holder HR4-707

    • CryoCane HR4-709

      Organized storage of CrystalCaps

Hampton 蛋白结晶向导管Wizard Tube

发表于由

Wizard Tube 
The Wizard™ Classic line of random sparse matrix screens is designed to increase your probability of producing crystals during the coarse screening phase when crystallizing biological macromolecules (proteins, nucleic acids, peptides, and combinations thereof).
The Wizard Classic reagents are proven to be a highly effective starting point in the screening of biological macromolecules. The Wizard Classic formulations include a large range of crystallants, buffers, and salts covering a broad range of crystallization space at pH levels from pH 4.5 to pH 10.5.Choose from Wizard Classic 1, 2, 3, or 4 non-overlapping formulations in matrix blocks or tubes.

图片

Wizard Cubic LCP
The tools in the Wizard Cubic LCP (lipidic cubic phase) Kit enable researchers to prepare LCP-type crystallizations by hand. Ideal for low-protein experiments: effective protein volume for a single crystallization experiment is about 80 nanoliters. Wizard Cubic LCP Kit tools work especially well when traditional methods have failed to yield crystals. Lipidic cubic phase has worked well for the crystallization of 7TM membrane proteins (proteins with seven transmembrane helices). Four out of six GPCRs (G-protein coupled receptors), an important membrane protein class, and several microbial 7TM proteins have been crystallized using the LCP approach.

图片

CRYO (I、II)
The Wizard Cryo™ line of random sparse matrix screens is designed for scientists who want to avoid the additional step of optimizing a cryoprotectant condition. Every Wizard Cryo formulation flash-freezes to a clear, amorphous glass in liquid nitrogen or in a cryo-stream at 100K. Crystals can be frozen directly from their growth drops, avoiding the additional step of pre-equilibration with an artificial cryo-condition that can damage the crystal. Eleven different cryocrystallants and sparing use of glycerol ensures a broad sampling of possible cryo conditions. Choose from Wizard Cryo 1 or 2 formulations in tubes or Wizard Cryo 1 and 2 together in a 96-well matrix block.

图片

Hampton Optimize Reagents

发表于由

Hampton Optimize Reagents

1、Optimize – Polymers

Crystallization grade polymers for formulating screens or optimization

2、Optimize – Organics (Volatile)

Crystallization grade organics (volatile) for formulating screens or optimization

3、Optimize – Organics (Non-Volatile)

Crystallization grade organics (non-volatile) for formulating screens or optimization

4、Optimize – Salts

Crystallization grade salts for formulating screens or optimization

5、Optimize – Buffers

Crystallization grade buffers for formulating screens or optimization

6、Optimize – Solubilizing Agents (NDSB)

Crystallization grade non detergent sulfobetaines

7、Optimize – Reducing Agent

TCEP Hydrochloride

8、Optimize – Monoolein

LCP – Lipidic Cubic Phase

9、Optimize – Cryoprotectants

For cryoprotection of crystals

10、Optimize – Oils for Crystallization

Microbatch crystallization & oil layering

11、Optimize – Izit Crystal Dye

Izit™ color dye to differentiate protein crystals from salt crystals

Hampton 蛋白结晶优化试剂 Optimization Screens

发表于由
Hampton 蛋白结晶优化试剂 Optimization Screens

1、Solubility & Stability Screen  蛋白特异性试剂优化溶解性和稳定性

Protein specific agents for optimization of solubility & stability  

2、Solubility & Stability Screen 2   离子缓冲液

Buffer, pH & ionic strength screen for optimization of solubility & stability

3、Slice pH    PH缓冲液

pH & buffer screen for optimization of crystals or sample solubility & stability

4、Additive Screen  96孔板添加剂试剂盒

96 compound screen for optimization of crystal size & quality

5、Silver Bullets • Silver Bullets Bio

Small molecule additive screen for optimization of crystal size & quality

6、Detergent Screen

Detergent screen for optimization of crystals or sample solubility & stability

7、Ionic Liquid Screen

Ionic liquid additive screen for optimization of crystal size & quality

8、StockOptions Buffer Kits

Titrated buffer sets for optimization of crystals or sample solubility & stability

9、StockOptions Polymer

n of crystal size & quality

10、StockOptions Salt

Salt reagent kit for optimization of crystal size & qualit

11、Polymer reagent kit for optimizatioy

Proti-Ace

In situ proteolysis kit protein for crystallization screening & optimization

12、Reductive Alkylation

Reductive alkylation kit for crystallization screening & optimization

13、Silica Hydrogel Kit

pH neutral silica hydrogel matrix crystallization

14、Low Melting Agarose

Agarose gel matrix crystallization

15、CryoPro

Water soluble cryoprotectant reagent set

16、Heavy Atom Screens

Heavy atom kits for multiple isomorphous replacement

17、I3C Phasing Kit

Produce heavy atom derivative of biological macromolecules for phasing